1 1 diphenyl 2 picryl hydrazyl radical dpph  (MedChemExpress)

Journal: Materials Today Bio

Article Title: Alchemizing earth's legacy: Bismuth-engineered humic nanoparticles for IBD theranostics through mitochondrial anti-inflammation and sustained intestinal delivery

doi: 10.1016/j.mtbio.2025.101948

Figure Lengend Snippet: Cellular uptake, ROS scavenging ability, and anti-inflammatory effects of BiHANs. A, B) Cellular uptake of BiHANs in RAW 264.7 cells visualized via fluorescence microscopy and bio-TEM after 24 h of treatment. Scale bar: 50 μm. C) Quantitative analysis of the DPPH (100 μM) scavenging capacity of BiHANs at different concentrations. D) Quantitative analysis of the H 2 O 2 (1 mM) scavenging capacity of BiHANs at different concentrations. E) Viability of H 2 O 2 -treated Caco-2 cells following various treatments. F) Schematic illustration of the ROS scavenging assay in H 2 O 2 -stimulated cells. G) Merged images of DCFH-DA (a ROS probe) staining (green) and DAPI staining (blue), along with fluorescence intensity analysis of RAW 264.7 cells under different treatment conditions. Scale bar: 50 μm. H) JC-1 fluorescence images (showing JC-1 monomers and aggregates) depicting mitochondrial membrane potential (Δψm) under different treatments, with corresponding fluorescence intensity quantification (mean ± SD, n = 3, ∗p < 0.05 , ∗∗p < 0.01 , ∗∗∗p < 0.001 , ∗∗∗∗p < 0.0001 ). I) RT-qPCR analysis of IL-1β , TNF-α and IL- 6 mRNA levels in RAW 264.7 cells after the indicated treatments. (J) Western blot analysis of iNOS and GAPDH protein expression in RAW 264.7 cells under different treatment conditions. (K) Schematic illustration of the LPS-induced RAW 264.7 cell polarization assay. Data were analyzed by Holm-Sidak's multiple comparisons test (E) and unpaired, two-sided Student's t-test (G–I). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The ability of BiHANs to scavenge DPPH free radicals was evaluated using the DPPH assay (TargetMol).

Techniques: Fluorescence, Microscopy, Staining, Membrane, Quantitative RT-PCR, Western Blot, Expressing

Journal: Food Chemistry: X

Article Title: Evaluation of pigment composition and antioxidant properties in the flesh of seven colored pummelo cultivars ☆

doi: 10.1016/j.fochx.2025.102705

Figure Lengend Snippet: The Pearson correlation analysis of major pigments and antioxidant capacities (HRSA, FRAP and DPPH). (A) Correlation analysis with carotenes. (B) Correlation analysis with flavonoids. (C) Correlation analysis with anthocyanins. The color scale in the right corner shows Pearson correlation from −1 (green) to 1 (red). ‘ * ’, ‘ ** ’, and ‘ *** ’ indicate p values <0.05, 0.01, and 0.001, respectively. Abbreviations: HRSA, Hydroxy free radical scavenging activity; FRAP, Ferric reducing antioxidant power; DPPH, 2,2′-diphenyl-1-picrylhydrazyl free radical scavenging capacity; VD, violaxanthin dibutyrate; VM, violaxanthin myristate; VMC, violaxanthin-myristate-caprate; VML, violaxanthin-myristate-laurate; A7G, apigenin 7-glucoside; K3N, kaempferol 3-neohesperidoside; I3N, isorhamnetin-3-O-neohespeidoside; I3G, isorhamnetin 3-O-glucoside; 6 A, 6- O -acetylglycitin; 7H4C, 7-hydroxy-4H-chromen-4-one; Q3G, quercetin-3-O-glucoside; C3G, cyanidin-3-O-glucoside; C3Ga, cyanidin-3-O-galactoside; Pel3G, pelargonidin-3-O-glucoside; D3G, delphinidin-3-O-glucoside; Pet3G, petunidin-3-O-glucoside; C3CG, cyanidin-3-O-(6-O-p-coumaroyl)-glucoside; C3CGa, cyanidin-3-O-(6”-O-coumaryl)galactoside; P3CG, pelargonidin-3-O-(6-O-p-coumaroyl)-glucoside; D3CG5Ga, delphinidin-3-O-(coumaroyl)glucoside-5-O-galactoside; P35CdG, Petunidin-3-O-5-O-(6-O-coumaroyl)-diglucoside. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Hydroxyl radical scavenging activity (HRSA), 2,2′-diphenyl-1-picrylhydrazyl free radical scavenging capacity (DPPH), and ferric reducing antioxidant power (FRAP) were determined using the micro HRSA assay kit (BC1325, Solarbio, China), FRAP assay (BC1315, Solarbio, Beijing, China), and DPPH free radical scavenging ability assay kit (BC4750, Solarbio, Beijing, China), respectively, according to the manufacturers' guidelines.

Techniques: Activity Assay